We then performed pathway enrichment analysis and revealed that the most significantly enriched pathways included Chagas disease, Kaposi Sarcoma-associated herpesvirus infection, IL-17 signalling pathway, AGE-RAGE signalling pathway, TNF signalling pathway, neurotrophin signalling pathway, apoptosis, and MAPK signalling pathway (Fig. 2B). The gene discussed is BDNF; the disease is Chagas disease.