In addition, we found that tumor cells of subtype2 was significantly enriched in PI3K-AKT, MAPK, TNF, and TGFB pathways, (Fig. 4), and the effect of inhibitors of these pathways on CRC cells vitality was verified by CCK8 experiments (Additional file 7: Fig. S4A). This evidence concerns the gene AKT1 and neoplasm.