Therefore, we applied RT-qPCR to examine FAM83H-AS1 expression and observed a markedly higher expression of FAM83H-AS1 in HCC cell lines (HepG2, Huh7, MHCC-97H and HCCLM3), particularly in the latter two (Fig. 1A). The gene discussed is SACK1H; the disease is hepatocellular carcinoma.