Therefore, in the present study, the expressions of mTOR and Ki-67 were detected in CSCC, BD, and AK tissues, the correlation between the expressions of mTOR and Ki-67 was analyzed, and the role of mTOR in the above three kinds of skin diseases with squamous cell differentiation abnormality and its relationship with the degree of tumor proliferation were explored, so as to provide new ideas for the diagnosis and treatment of CSCC. The gene discussed is MKI67; the disease is skin squamous cell carcinoma.