Treatments with 15 μg/ml (5.03 μM) of TP4 are sufficient to kill over 50% of breast cancer cells at 6 h.Lactate dehydrogenase (LDH) increased at 3 h post-TP4 treatment in TNBC cells, indicating that peptide induces necrotic death in TNBC cells.TP4 binds to the mitochondria, disrupts Ca2+ homeostasis, and ultimately induces FOSB protein. This evidence concerns the gene FOSB and breast cancer.