RPL9 KD inactivates Id-1/nuclear factor-κB (NFкB) signaling pathway in HT29 and HCT116 colon cancer cells when compared with the control by phosphorylating IкB, which is known to prevent the translocation of NFкB into the nucleus and the activation of genes that promote cell survival. The gene discussed is RPL9; the disease is malignant colon neoplasm.