Further, oxidative bisulfite sequencing demonstrated increased levels of 5hmC in these regions in AML samples that were wild type for TET2, IDH1, and IDH2; 5hmC levels were significantly lower in IDHmut or TET2mut samples in these regions, providing evidence that these mutations cause increased DNA methylation by impairing TET-mediated DNA demethylation. Here, TET2 is linked to acute myeloid leukemia.