Specifically, Pharo et al. detected single methylated concentration of SEPT9 and CDO1 in several CRC cell lines through ddPCR and observed that Colo205, Colo320, and HCT116 had higher SEPT9 methylated concentration than the other cell lines, with the lowest level in SW480, which was in accordance with our observation24. This evidence concerns the gene SEPTIN9 and colorectal carcinoma.