We have shown Kupffer cell frequency drastically decreases after IR stress, with a simultaneous hepatic influx of circulating CD11b+ macrophages.30 Notably, we have detected upregulated Ikaros levels in liver-infiltrating CD11b+ cells, coinciding with enhanced pyroptosis (GSDMD) but negatively associated with SIRT1, in a murine LPS/D-Galactosamine hepatitis model (Fig. S7). Here, SIRT1 is linked to hepatitis A virus infection.