To determine whether β-arrestin–mediated AT1R activation had beneficial effects on survival, rats were injected with MCT to induce PAH, and after 2 weeks minipumps were implanted for treatment with AngII, TRV023, or losartan for up to 4 weeks via minipump (Figure 1H). The gene discussed is AGTR1; the disease is pulmonary arterial hypertension.