In order to validate 2D-GE and LC/ESI-MS/MS data, we performed ELISA analyses for AO, C5, and TF in naturally exposed cattle to M. bovis, infected (bTB+) and not infected (bTB-) and with and without a concomitant infectious or metabolic disease (IMD), as well as in healthy cattle (HS) not exposed to M. bovis. This evidence concerns the gene AOX1 and Other metabolic disease.