When stimulated, CAF shows the potential to differentiate into adipocytes, osteoblasts, and pancreatic cells. When co-cultured with human HCC cell lines, CAF upregulated the expression of TGFB1 and FAP genes in Huh-7 and JHH-6, thus having the ability to enter the circulation. This evidence concerns the gene TGFB1 and hepatocellular carcinoma.