Western blotting assay results showed that the expression of apoptosis-related proteins Cyt C, cleaved caspase 3, 57 and 67 kDa AIF and that of cellular senescence-associated proteins P53, P21, and P16 in HF-MSCs overexpressing PBX1 was significantly lower than that of the control or empty vector group (p < 0.05; Figures 2D,G,H). The gene discussed is TP53; the disease is hydrops fetalis.