CASP3 and hydrops fetalis: In agreement with ROS, senescence, and apoptosis assay results, the western blotting assay showed that PBX1 overexpression in H2O2-treated and -untreated HF-MSCs significantly reduced the expression of 1) senescence-related proteins P53, P21, and P16 (p < 0.05; Figures 5E,G); 2) DNA damage and repair-related proteins γH2AX, OGG1, Rad51, KU70, Ku80, PAR, and 116 kDa PARP1 (p < 0.05; Figures 5E,I,J); and 3) apoptosis related proteins 57 and 67 kDa AIF, cleaved caspase 3, and Cyt C (p < 0.05; Figures 5E,F).