Overall, our results showed that PBX1 overexpression attenuated cellular senescence and apoptosis in HF-MSCs by alleviating ROS-mediated DNA damage and repair, providing new insight into the mechanistic understanding of cellular senescence and apoptosis, and significantly contributing to the future development of strategies for alleviating tissue and organ aging, and, in particular, hair regeneration. This evidence concerns the gene PBX1 and hydrops fetalis.