CDK1 and gastric cancer: The flow cytometry results suggested that Cos significantly induced cell cycle arrest in the G2/M phase in HGC-27, SNU-1 cells with obvious dose-dependency (p < 0.001), but the effect of Cos on GES-1 was not as sensitive as GC cells (p > 0.05) (Figure 2A), and Western blot showed that the expression levels of cell cycle-related proteins (Cdc25c, Cdk1, Cyclin B1) in GC cells were significantly downregulated by Cos, especially in the 40 μM Cos group (p < 0.001), but the effect of Cos on GES-1 cells was not significant (p > 0.05) (Figure 2B).