Consistent with the gene expression in this study, and protein levels within a previous study (Landberg et al., 2020a), 3D cultured cells had upregulated protein levels of markers for cancer stemness (CD44) and pluripotency (POU5F1), and downregulated levels of markers for proliferation (CCNA2) and differentiation (ERS1). The gene discussed is POU5F1; the disease is cancer.