3D cultured cells were also shown to have lower levels of genes related to cell proliferation or mitosis (CCNA2, MKI67, and BUB1) and differentiation (ESR1) relative 2D cultured, consistent with higher levels of a cell cycle inhibitor (CDKN1) and cancer stemness–related markers (CD44 and MALAT1). The gene discussed is CCNA2; the disease is cancer.