The results showed that SPP1, GOT2, and EHHADH were significantly associated with immune score or stromal score (Figure 9G).Then, the role of the six MRGs in the tumor immune microenvironment in HCC was examined by assessing the correlation between the expression of the six MRGs and infiltrated immune cell types and tumor purity using the TIMER database. The gene discussed is SPP1; the disease is hepatocellular carcinoma.