The results showed that high expression of WFS1 was closely associated with PDCD1, LAG3, TIM3, OX40, TIGIT, and CD276 in HCC, and low expression of EHHADH was negatively correlated with PDCD1, LAG3, TIM3, OX40, TIGIT, CD276, and CTLA-4. This evidence concerns the gene CD276 and hepatocellular carcinoma.