MRAP and colorectal carcinoma: To investigate the effect of Berberine on CRC-derived stem-like cells, we enriched spheres from HCT116 and HT29 cells by maintaining them in ultra-low-attachment dishes in serum-free and stem cell-inducing medium (DMEM/F12 supplemented with B27, EGF, and bFGF) (Figure 1A).