U87 and GL261 glioblastoma cell lines showed similar 18F-PSMA-1007 uptake to PSMA negative PC-3 cells for all densities and incubation times, with maximum uptake of 0.59% for PC-3 (60 min,1 x 106 cells per well), 0.64% for U87 (120 min, 6 × 105 cells per well) and 0.73% for GL261 (60 min, 1 x 106 cells per well). Here, FOLH1 is linked to glioblastoma.