As shown in Figure 1A, the level of ACSL4 expression positively correlated with immune purity (R = 0.194, P = 1.76e-04) and the infiltration levels of specific subsets of immune cells, including B cells (R = 0.071, P = 1.78e-01), CD8+ T cells (R = 0.192, P = 2.22e-04), CD4+ T cells (R = 0.086, P = 1.00e-01), macrophages (R = 0.13, P = 1.27e-02), neutrophils (R = 0.3, P = 5.30e-09), and dendritic cells (R = 0.2140, P = 3.87e-05) in BLCA. The gene discussed is ACSL4; the disease is bladder transitional cell carcinoma.