To further verify that CAFs promote the immune escape of colorectal cancer cells by upregulating the expression of PD-L1 through Akt phosphorylation, we cultured CRC cells with CAF-CM, CAF-CM + pAKTi, and normal medium, and the rate of killing by PBMCs was detected after 3 days. Here, AKT1 is linked to colorectal carcinoma.