Univariate Cox regression analysis indicated that POLD1, pTNM staging and grade were closely related to the survival of HCC patients (P<0.05), and multivariate Cox regression revealed the significant effects of POLD1 expression (HR=1.54, P=0.0005) and pTNM staging (HR=1.27, P=0.016) on the prognosis of HCC (Figures S3A, B). The gene discussed is POLD1; the disease is hepatocellular carcinoma.