To further explore its mechanism, we overexpressed the LTF gene in PRAD PC-3 cells and verified the effect of high expression of LTF on JAK/STAT3 pathway-related proteins and PRAD-derived GM-CSF proteins by Western blot analysis, RT-qPCR, and enzyme-linked immunosorbent assay. This evidence concerns the gene LTF and prostate adenocarcinoma.