Specifically, flow cytometry showed that DIO mice were characterized by substantially higher counts of tumor-infiltrating CD8+ T cells (CD8+ TILs) expressing PD-1, T-cell immunoglobulin mucin-domain containing 3 (Tim3), and lymphocyte activating gene 3 (Lag3), and lower proliferation by Ki67 than control mice. The gene discussed is CD8A; the disease is neoplasm.