Moreover, we found that shorter incubation times with IFNλ3 followed by its removal before infection reduced its antiviral activity to a greater extent compared to the three IFNλ4 variants used in the experiment (compare early time points in Figure 4B with Figures 4C–E); comparison of activity between washed and unwashed was significant (t-test) at all time points for IFNλ3, whilst for IFNλ4s, it was only significant for the first or second time points. The gene discussed is IFNL4; the disease is infection.