Investigating these clusters in Kaluza (software version 2.1) revealed an association between tumor stage and CD279- naïve CD8+ T cells (Figure 8F), CD279+ CD8+ TCM (Figure 8G), CD279+ CD8mid TEM (Figure 8H) and CD279+ CD8mid TCM (Figure 8I) as well as CD279high CD4+ TCM (Figure 8J). This evidence concerns the gene PDCD1 and neoplasm.