STAT1 and Sepsis: Interestingly, when we tested the set of hypomethylated CpGs in LPS-treated monocytes (Hypo-CpGs) with STAT1/3/5 binding motifs with the DNA methylation data of the sepsis and healthy donor’s cohort, we found lower methylation levels in gram-negative sepsis patients in comparison with any of the other groups (Figure 4A), reinforcing the notion of the participation of the JAK2-STAT pathway in the first encounter with gram-negative bacteria under in vivo conditions.