Consistent with the exhausted phenotype displayed by the tumor-specific TILs (Figures 2A and S3D, E), IFN-γ+ TNFα+ IL-2+ tumor-specific T cells were undetectable in MC38 tumors, and on average made up only 15% of tumor-specific T cells isolated from B16.F10 tumors. This evidence concerns the gene TNF and neoplasm.