Western blot analysis showed that the protein levels of TLR4, p-NF-κB (p-p65 and p-IKβα), and MyD88 in kidney tissues were significantly increased in CKD compared to CTL, while BYF treatment restored these proteins with the high doses effect being generally more evident (Figures 5C,D). The gene discussed is NFKB1; the disease is chronic kidney disease.