To determine whether ETS1 is upregulated in GBM ECs via TGFβ-dependent manner, bEND.3 cells were stimulated with CT-2A glioma cells conditioned medium with neutralizing antibody against TGFβ or control antibody, after which the expression of Ets1 was analyzed by qPCR. This evidence concerns the gene TGFB1 and central nervous system cancer.