To determine whether ETS1 is upregulated in GBM ECs via TGFβ-dependent manner, bEND.3 cells were stimulated with CT-2A glioma cells conditioned medium with neutralizing antibody against TGFβ or control antibody, after which the expression of Ets1 was analyzed by qPCR. This evidence concerns the gene TGFB1 and glioblastoma.