To elucidate the effect of eIF5A2 on PC metastasis in vivo, we transfected 22RV1 cells with Lenti-Luciferase-Zsgreen-Puro and then with NC, eIF5A2 siRNA, or eIF5A2 plasmid and observed lung metastasis in vivo. The gene discussed is EIF5A2; the disease is pachyonychia congenita.