To interrogate the effect of IL-22 signaling on glycosyltransferase gene expression in human intestinal epithelial cells, we used differentiated Caco-2 BBe1 cells, a subclone of the Caco-2 human colorectal adenocarcinoma cell line that displays small-intestine-like morphology and biochemical properties (17, 18). Here, IL22 is linked to colorectal adenocarcinoma.