To explore the function of RORγt in the effects of anti-miRNA-221-5p on Th17/Treg Ratio in asthma, si-RORγt was used suppress the protein expression of RORγt and induced that of FOXP3 in in vitro model of asthma following anti-miRNA-221-5p, compared with anti-miRNA-221-5p group (Fig. 7A–C). Here, FOXP3 is linked to asthma.