IDH1 and central nervous system cancer: As desired, this threshold divided histological lower-grade IDH-wildtype gliomas without EGFR amplification into two groups with significantly different age distributions (median age 35 vs. 59.5, p < 0.0005, Mann–Whitney U-test), especially when TERTp mutation status was accounted for (median age 26) (Fig. 2D, Additional file 2: Fig. 3B, C).