we performed immunohistochemistry to examine the degree of protein expression and found that hypomethylation of the above four genes were linked to a higher protein staining level (Fig. 6B, E, H, K), Statistical analysis also showed that the pregnant group had higher levels of TBX3, AKT3, SOX2, and SGK1 than non-pregnant adenomas group, with a significant difference (P < 0.05 or P < 0.01) (Fig. 6C, F, I, L). The gene discussed is SOX2; the disease is adenoma.