PI3K-AKT pathway, JAK-STAT pathway and MAPK pathway can potentially be the downstream of IL4/IL4R signal (Heredia et al. 2013; Keijzer et al. 2011; Malabarba et al. 1995; Friedrich and Wietek 2001), we revealed that the blockage of ERK pathway most significantly inhibited the IL-4-mediated proliferation of early OCPs, while AKT pathway also partially regulated the proliferation, indicating IL-4 regulated the proliferation of early OCPs during bone metastasis of CRC through several ways and ERK pathway could be the most important regulator. This evidence concerns the gene IL4R and colorectal carcinoma.