Corroborating the progressive loss of MGMT expression in HCC progression, immunohistochemical analysis of MGMT in an independent set of 12 matched CLD and HCC samples showed significant reduction in MGMT expression in HCC compared with matched CLD samples (P = 0.03, Wilcoxon’s test, Fig. 3C). This evidence concerns the gene MGMT and congenital secretory chloride diarrhea 1.