In this study, the results of nuclear cytoplasmic protein and cell cycle assay confirmed that in p53 wild type colorectal cancer cell line, CCT8 inhibited p53 entry into the nucleus, down-regulated cell cycle inhibitory proteins p21 and P27, and up-regulated cell cycle-related molecules (CDK4, CDK6, cyclinD1, myc), and promote tumor cell proliferation. This evidence concerns the gene TP53 and colorectal cancer.