We also stimulated splenocytes and tumor-infiltrating cells with peptide Ags in vitro and determined a markedly higher frequency of IFN-γ+ cells in the CD8+ T-cell population in response to Nes2LR in the spleen and tumor from mice treated with CPQ/Nes2LR vaccination compared with untreated and 2HPQ/Nes2LR vaccinated mice (online supplemental figure S10 and figure 4H). The gene discussed is CD8A; the disease is neoplasm.