To investigate the regulatory role of baicalin in autophagy and PI3K/AKT signaling in glaucoma mice, the expression of autophagy-related proteins (LC3-I, LC3-II, Beclin-1, and ATG5) and PI3K/AKT signaling-associated proteins (pAKT, AKT, pPI3K and PI3K) in retinal tissues of four groups (control, model, Model+NS and Model+BAI) was detected by Western blotting. This evidence concerns the gene ATG5 and glaucoma.