After LIFU, there is a release and elevation of heat-shock protein 70, IL-8, TNF-α, and damage-associated molecular patterns in the parenchyma.82 Aravantis et al examined the effect of focused ultrasound with the uptake of 2 relevant chemotherapies, doxorubicin and ado-trastuzumab emtansine83 in a HER2 amplified estrogen-dependent model of breast cancer brain metastasis. The gene discussed is ERBB2; the disease is breast cancer.