Based on GDSC data, we performed the analysis to find out the association between PBK expression levels with IC50 values of different drugs in pan-cancer, and we found that the PBK expression level was positively correlated with trametinib, RDEA119, PD032590, and selumetinib, which indicated that PBK may be positively correlated with the treatment of these chemotherapeutic drugs and may provide a new direction in tumor chemotherapy. This evidence concerns the gene PBK and neoplasm.