Since the tripartite interaction between FnBPs expressed by S. aureus, fibronectin molecules, and the α5β1 integrin expressed at the surface of eukaryotic cells is acknowledged as the major pathway driving the internalization of S. aureus in NPPCs, we decided to investigate if S. aureus clinical isolate strains recovered from keratitis could trigger their internalization by corneal epithelial cells. Here, FN1 is linked to keratitis.