After that, the possible mechanism of the high expression of ETS-1 in astrocytoma was initially explored, and a series of research techniques (Transwell, nude mouse subcutaneous tumor formation experiment) were used to detect the invasion of U87 and U251 cells in vitro by ETS-1 and its upstream pathways and the influence of these factors was also revealed in tumor formation of nude mice. The gene discussed is ETS1; the disease is neoplasm.