These five biological processes included 32 DMR-associated genes (Supplementary Table 4), of which seven genes, including TRIO(high confidence),DCC(strong candidate), PLXNA4(strong candidate), PLXNB1(strong candidate), GLRA2(suggestive evidence), NRP2(suggestive evidence), and PLXNA3(suggestive evidence), were autism candidate genes reported in the SFARI database (https://gene.sfari.org/database/human-gene/).To avoid enrichment biases derived by gene length, genes with DMRs in promoters were analyzed by GO. The gene discussed is TRIO; the disease is autism.