To further validate whether Kcnq1ot1 affects AKI development by mediating the miR-204-5p/NLRP3 axis, mice were injected with the Kcnq1ot1 knockdown vector one week before establishing the AKI model and transfection efficiency was detected by RT-qPCR (Figure 6A). This evidence concerns the gene NLRP3 and acute kidney injury.