To further investigate the functional mechanism of Kcnq1ot1 in AKI, the subcellular localization of Kcnq1ot1 was identified and the result showed that Kcnq1ot1 was mainly located at the cytoplasm of HK-2 cell, which suggested that Kcnq1ot1 may competitively bind to a miRNA in HK-2 cells to form a ceRNA regulatory network. This evidence concerns the gene KCNQ1OT1 and acute kidney injury.