Univariant analysis revealed that serum total bilirubin, direct bilirubin, glutamate transpeptidase, nucleotidase, total bile acid, cholinesterase, fucosidase, cystatin C, creatinine and uric acid were statistically significant risk factors for survival in advanced HCC patients (Supplementary Table S2). The gene discussed is CST3; the disease is hepatocellular carcinoma.