SMAD4 and colon adenocarcinoma: However, using The Cancer Genome Atlas (TCGA) database of colon adenocarcinoma (COAD) transcriptomes, we found that SMAD4mut tumors are not underrepresented among CMS4 samples when compared to other subtypes or all tumors in the database (Fig. 7b), raising the possibility that SMAD4 is also not obligatory for EMT in vivo.