Targeting Pten and p53 resulted in tumor formation identified by histopathological HE staining, no expression of Pten, and overexpression of mutant p53 (It should be noted that the physical and chemical properties of mutant p53 are different from wild-type p53.33 Normally, the mutant p53 has higher products in proliferating cells, better stability and longer half-life (1.4–7 h), which can be detected by IHC. The gene discussed is PTEN; the disease is neoplasm.