In the present study, cerebral ischemia resulted in downregulation of the Wnt-3a and β-catenin proteins, while LMP2 inhibition rescued Wnt-3a and β-catenin protein expression in the LMP2-shRNA group compared with the control-shRNA group (P < 0.001) (Fig. 1d). This evidence concerns the gene PSMB9 and brain ischemia.