Therefore, we decided to validate whether PKMYT1AR, miR-485-5p or/and PKMYT1 could be used as novel therapeutic targets for NSCLC in the future, and decipher how PKMYT1AR/miR-485-5p/PKMYT1 axis regulates NSCLC progression both in vitro and in vivo. The gene discussed is PKMYT1; the disease is non-small cell lung carcinoma.